This workflow uses refseq_masher and Mash to find what NCBI RefSeq Genomes match or are contained in some input sequence data. The specific Galaxy tools are listed in the table below.

Tool Name Owner Tool Revision Toolshed Installable Revision Toolshed
refseq_masher nml 26df66c32861 0 (2018-02-15) Galaxy Main Shed

To install these tools please proceed through the following steps.

Step 1: Galaxy Conda Setup

Galaxy makes use of Conda to automatically install some dependencies for refseq_masher. Please verify that the version of Galaxy is >= v16.01 and has been setup to use conda (by modifying the appropriate configuration settings, see here for additional details). A method to get refseq_masher to work with a Galaxy version < v16.01 is available in FAQ/Conda dependencies.

Step 2: Install Galaxy Tools

Please install all the Galaxy tools in the table above by logging into Galaxy, navigating to Admin > Search and browse tool sheds, searching for the appropriate Tool Name and installing the appropriate Toolshed Installable Revision.

The install progress can be checked by monitoring the Galaxy log files galaxy/*.log. On completion you should see a message of Installed next to the tool when going to Admin > Manage installed tool shed repositories.

Step 3: Testing Pipeline

A Galaxy workflow and some test data has been included with this documentation to verify that all tools are installed correctly. To test this pipeline, please proceed through the following steps.

  1. Upload the refseq_masher Galaxy Workflow by going to Workflow > Upload or import workflow.
  2. Upload the sequence reads by going to Analyze Data and then clicking on the upload files from disk icon upload-icon. Select the test/reads files. Make sure to change the Type of each file from Auto-detect to fastqsanger. When uploaded you should see the following in your history.


  3. Construct a dataset collection of the paired-end reads by clicking the Operations on multiple datasets icon datasets-icon. Please check off the two .fastq files and then go to For all selected… > Build List of dataset pairs. You should see a screen that looks as follows.


  4. This should have properly paired your data and named the sample AE014613-699860. Enter the name of this paired dataset collection at the bottom and click Create list.
  5. Run the uploaded workflow by clicking on Workflow, clicking on the name of the workflow RefSeqMasher-sequence_reads_paired-v0.1.0 (imported from uploaded file) and clicking Run. This should auto fill in the dataset collection. Click Run workflow.


  6. If everything was installed correctly, you should see each of the tools run successfully (turn green). On completion this should look like.


    refseq_masher matches output:


    refseq_masher contains output:


    If you see any tool turn red, you can click on the view details icon view-details-icon for more information.

If everything was successfull then all dependencies for this pipeline have been properly installed.